diff --git a/modules/videoio/src/cap_ffmpeg_impl.hpp b/modules/videoio/src/cap_ffmpeg_impl.hpp
index 33f6eecc70..b892bd561b 100644
--- a/modules/videoio/src/cap_ffmpeg_impl.hpp
+++ b/modules/videoio/src/cap_ffmpeg_impl.hpp
@@ -1105,7 +1105,7 @@ bool CvCapture_FFMPEG::open(const char* _filename, int index, const Ptr<IStreamR
                 {
                     enableAlpha = false;
                 }
-                if (value == CV_8UC4)
+                else if (value == CV_8UC4)
                 {
                     enableAlpha = true;
                 }
diff --git a/modules/videoio/test/test_ffmpeg.cpp b/modules/videoio/test/test_ffmpeg.cpp
index a0b53219d6..0854dec433 100644
--- a/modules/videoio/test/test_ffmpeg.cpp
+++ b/modules/videoio/test/test_ffmpeg.cpp
@@ -845,6 +845,22 @@ TEST(videoio_ffmpeg, create_with_property_badarg)
     EXPECT_FALSE(cap.isOpened());
 }
 
+TEST(videoio_ffmpeg, open_with_format_cv8uc3)
+{
+    if (!videoio_registry::hasBackend(CAP_FFMPEG))
+        throw SkipTestException("FFmpeg backend was not found");
+
+    string video_file = findDataFile("video/big_buck_bunny.mp4");
+    VideoCapture cap(video_file, CAP_FFMPEG, {
+        CAP_PROP_FORMAT, CV_8UC3
+    });
+    ASSERT_TRUE(cap.isOpened());
+    EXPECT_EQ(cap.get(CAP_PROP_FORMAT), CV_8UC3);
+    Mat frame;
+    ASSERT_TRUE(cap.read(frame));
+    EXPECT_EQ(frame.channels(), 3);
+}
+
 // related issue: https://github.com/opencv/opencv/issues/16821
 TEST(videoio_ffmpeg, DISABLED_open_from_web)
 {